Frequent social regroupings affect corporal and reproductive development, metabolic status, and behavioral time budget in dairy heifers.

We aimed to evaluate the corporal and reproductive development, metabolic status, and behavioral patterns of resident dairy heifers subjected to social regroupings (SR). Holstein replacement heifers (153.3 ± 16.1 kg, 11 months old) were homogeneously allocated to: 1) Control (CON; n = 14 resident + 5 "permanent") and 2) Regrouped (RG; n = 14 resident + 5 "changing"). "Changing" heifers were exchanged for 5 novel heifers every 21 d over 205 days (total = 10 SR). At each SR, body weight, wither height, body condition score (BCS), eating, ruminating, lying and standing behaviors were recorded, and blood hormones and metabolites were determined. Behavior was registered through scan sampling every 10 min., and estrus was detected using patches. Wither height and BCS were greater in CON than RG at SR9 and 10, and at SR4 and 5, respectively (P < 0.01). Control heifers showed first heat earlier (93 ± 9 d vs 126 ± 14 d; P = 0.03) and presented greater insulin growth factor- 1 (171.3 vs 120.0 ng/ml ± 10.1; P < 0.01) and glucose (4.3 vs 4.2 mmol/L ± 0.07; P = 0.04) concentrations than RG, while non-esterified fatty acids concentration was greater in RG (0.35 vs 0.30 mmol/L ± 0.04 P = 0.04). Regrouped heifers were observed more times eating (0.45 vs 0.33 ± 0.01), but fewer times ruminating (0.21 vs 0.24 ± 0.01) than CON (P < 0.01). Frequent SRs negatively affected corporal and reproductive development and energy metabolism, and altered behavioral patterns in dairy heifers.

Social dominance affects intake rate and behavioral time budget in pre-pubertal dairy heifers allocated in continuous competitive situations.

In intensive feeding systems, competition may be high and social dominance may affect animal performance by changing dry matter intake (DMI) and behavioral time budgets. If competition level is maintain over time, the strategies developed by heifers of different social status are expected to differ. Thus, the aim of this study was to compare individual DMI, intake rate and eating, ruminating, lying and standing behaviors in dominant (DOM) and subordinate (SUB) pre-pubertal dairy heifers in a model study implying continuous competitive situations. A total of 16 Holstein and Jersey×Holstein pre-pubertal heifers (251±10 days old, weighing 208±14 kg; mean±SEM) were allocated into eight homogeneous dyads. Each dyad was maintained during 120 days (day 0=beginning of measurements) in pens, and received a total mixed ration from one feeder/dyad. The DOM and SUB heifers was determined (day 0, twice during the first month of the experiment and every month afterwards) by observation of the winner in agonistic interactions in each dyad after the feed was supplied. The general activity pattern (eating, ruminating, lying and standing) of each heifer was recorded by direct instantaneous scan-sampling, every 10 min for 12 h, in 7 days (days 1, 21, 35, 60, 75, 100 and 120). Individual DMI was estimated with the double marker technique, in three intervals (I=days 17-26; II=days 78-87 and III=days 112-120), while estimated intake rate (kg/min) was calculated for each interval as the DMI per total eating time. After the experiment was concluded, data of the first 5 and the last 6 h of the 12 h scan-sampling (related to the moment the feed was supplied) was grouped according to the moments of greater and lesser competition for feed on each day. During the first 5 h, where competition was expected to be highest, no differences in eating behavior were found between heifers of different social status, but DOM heifers spent more time ruminating and lying than SUB heifers, while SUB spent more time standing than DOM heifers. No differences were found on DMI between DOM and SUB, but SUB ate at a faster rate on interval II compared with DOM heifers. In conclusion, in this model study of heifer dyads, SUB heifers had greater intake rate with no differences in feed intake, spent less time ruminating and lying, and more time standing than DOM heifers during the first hours after feed delivery.

Social dominance in prepubertal dairy heifers allocated in continuous competitive dyads: Effects on body growth, metabolic status, and reproductive development.

The objective of this study was to compare the body weight (BW) and size, metabolic status, and reproductive development of dominant and subordinate prepubertal dairy heifers allocated in competitive dyads. Sixteen Holstein and Jersey × Holstein prepubertal heifers (means ± SEM; 250.8 ± 9.8 d; 208.5 ± 13.9 kg of BW) were assigned to 8 homogeneous dyads according to breed, age, and BW. Dyads were housed in pens separated 1 m from each other during 120 d, receiving a total mixed ration on a 5% restriction of their potential dry matter intake, and had access to the same feeder (60 cm) throughout the experiment. Dominant and subordinate heifers were defined based on the winning agonistic interactions in each dyad. Body development was recorded every 20 d in all heifers, and blood samples were collected on the same days to determine endocrine and metabolic status. The maximum follicle diameter, number of follicles >6 mm, and the presence of corpus luteum were observed weekly by ultrasound. Heifer BW (269.3 vs. 265.3 ± 1.5 kg) and average daily gains (0.858 vs. 0.770 ± 0.02 kg/d) were greater in dominant than subordinate heifers. On d 30, 37, and 53, dominant heifers had more follicles than subordinate heifers, and maximum follicle diameter was greater in dominant than in subordinate heifers (10.0 vs. 9.0 ± 0.3 mm). Dominant heifers achieved puberty earlier than subordinate heifers (313.9 ± 4.9 vs. 329.6 ± 5.7 d) with similar BW (279.4 ± 2.6 vs. 277.4 ± 5.8 kg). Glucose concentrations were greater in dominant than subordinate heifers (89.2 vs. 86.8 ± 1.2 mg/dL), but cholesterol concentrations were greater in subordinate than dominant heifers (86.1 vs. 90.2 ± 2.6 mg/dL). We concluded that, under continuous competitive situations, dominant heifers were more precocious than subordinate ones, achieving an earlier puberty. Dominant heifers had greater body growth and glucose concentrations than subordinate heifers, which may be responsible, at least in part, for the differences on reproductive development between heifers of different social status.

Positive effects of biostimulation on luteinizing hormone concentration and follicular development in anestrous beef heifers.

The objectives of this study were to characterize the LH secretion pattern and the follicular development of anestrous beef heifers during early exposure (first 30 d of exposure) to androgenized steers (AS) and to determine if exposure to AS for 80 d (includes the first 30 d and 50 d more) advances the onset of ovarian cyclic activity. Twenty-nine anestrous Hereford heifers (20.2 ± 4.1 mo old and 257.5 ± 32.5 kg of BW) were allocated to 2 homogeneous groups according to their age and BW: 1) heifers exposed to AS (group EH; = 15) for 80 d and 2) control heifers, isolated from AS and any other male during all the course of the study (group CH; = 14). On d 0, 3 AS were joined with the EH group, which were removed and replaced with other 3 AS on Day 14. On d -10, 1, 10, 20, and 30, 8 heifers per group were housed in individual stalls and blood samples for LH were collected at 15-min intervals for 6 h. From d -10 to 30, the maximum follicle diameter (MFD) and the presence of a corpus luteum (CL) was daily recorded by ultrasound scanning and estrous behavior was detected twice daily. The emergence of follicular waves (FW), defined as the day when the dominant follicle of a wave was first observed (3-4 mm diam.), was retrospectively determined. Afterward, ultrasound scannings were performed weekly from d 32 to 60 and on d 70 and 80 to determine the presence of CL. After 10 d of male exposure, LH concentrations, either mean (1.67 vs. 0.88 ng/mL [SEM 0.09]) or basal (1.53 vs. 0.74 ng/mL [SEM 0.09]), were greater ( < 0.05) in the EH group than in the CH group. There was a treatment effect in MFD, as it was greater in EH than in CH ( = 0.05; 8.00 ± 0.16 vs. 7.52 ± 0.17 mm, respectively), but none of those follicles ovulated during the 40-d period. The MFD of the second FW was greater in EH than in CH, in coincidence with the transient increase on LH concentrations, which probably induced the greater follicular growth. Cumulative proportions of heifers that started to cycle were greater ( = 0.01) in EH than in CH on d 60 (33.3 vs. 0%), 70 (47 vs. 0%; < 0.005), and 80 (53 vs. 0%; < 0.001) of the exposure period. In conclusion, exposure of anestrous beef heifers to AS resulted in a transient increase on LH secretion after 10 d of male exposure and increased follicular diameter attained during the second FW. In addition, ovarian cyclic activity was advanced in exposed heifers.

Genetic selection for temperament affects behaviour and the secretion of adrenal and reproductive hormones in sheep subjected to stress.

We investigated the effect of genetic selection for temperament on the way that stressors affect the behaviour and the adrenal and reproductive axes of sheep. We tested three hypotheses: (i) isolation would increase cortisol secretion and decrease luteinising hormone (LH) secretion more in nervous sheep than in calm sheep; (ii) isolation combined with simulated human presence would increase cortisol secretion and decrease LH secretion more in nervous sheep than in calm sheep and (iii) isolation combined with stressors that were not specific to the selection process (i.e. non-selection stressors) would increase cortisol secretion and decrease LH secretion equally in calm and nervous sheep. Isolation alone increased cortisol secretion and decreased LH secretion in nervous sheep but not in calm sheep. Compared to calm sheep, nervous sheep were more agitated during the first 2 h of isolation but not during the second 2 h of isolation. Exposure to non-selection stressors increased cortisol secretion, decreased LH pulse amplitude and the mean plasma concentrations of LH in both calm and nervous sheep. We conclude that genetic selection for temperament affects the behavioural expression of the stress response and the secretion of adrenal and reproductive hormones during isolation, but has less impact on their reactivity to non-selection stressors.

Genetic differences in temperament determine whether lavender oil alleviates or exacerbates anxiety in sheep.

Growing concerns about the risk of addiction to benzodiazepines have led to increasing interest in alternative therapies to treat anxiety and depression. Lavender oil (Lavendula augustifolia) is reportedly anxiolytic in a number of species but little is known about how it affects individuals that are more or less anxious when faced with a stressor. In this study, we used changes in locomotor activity and the plasma concentrations of cortisol to test whether lavender oil would reduce behavioral and endocrine correlates of anxiety in calm and nervous sheep exposed to an isolation stressor. During the non-breeding season, 'calm' or 'nervous' female sheep from the UWA temperament flock were exposed to a mask containing either 1 mL of 10% lavender oil (calm: n=8; nervous: n=8) or peanut oil (calm: n=8; nervous: n=8). After 30 min, each sheep was isolated for 5 min and then returned to the group. Blood was sampled prior to the mask, prior to isolation, 1 min and 30 min after isolation to profile changes in the plasma concentrations of cortisol. Agitation score, locomotor activity and vocalizations were recorded as correlates of anxiety associated with the isolation stressor. Irrespective of whether they were exposed to lavender oil, calm sheep had a lower agitation score (P<0.001), crossed the central lines of the isolation box less frequently (P<0.001), expressed fewer vocalizations (P<0.001) and had lower plasma concentrations of cortisol immediately after isolation (P<0.001) than nervous sheep. Exposure of calm sheep to lavender oil decreased the agitation score (P<0.001), frequency of vocalizations (P<0.05), decreased the number of crosses of the central lines of the isolation box (P<0.05), and the plasma concentrations of cortisol prior to isolation (P<0.05) (after mask application) compared to calm control sheep. Exposure of nervous sheep to lavender oil increased the frequency of vocalizations (P<0.05), the number of sheep attempting to escape (P<0.05) and the plasma concentrations of cortisol 30 min after isolation (P<0.05) compared to nervous control sheep. We conclude that genetic differences in temperament determine whether lavender oil alleviates or exacerbates the behavioral and/or endocrine correlates of anxiety in sheep.

Response to biostimulation in peri-puberal beef heifers: influence of male-female proximity and heifer's initial body weight.

The objectives were to determine if exposure of 12 mo old peri-puberal beef heifers to androgenized steers for 35 d hastened puberty, and if the response was related to the physical proximity between males and females and to heifer's initial body weight. Hereford x Aberdeen Angus heifers (n = 131), 12 mo old, were assigned to two treatments: 1) Exposed group, exposed to androgenized steers from Day 0 to Day 35 (E, n = 66); or 2) Control group, isolated from the steers and other males (C, n = 65). Cyclic activity was determined through estrous behavior detection (twice daily) and weekly ultrasound imaging to detect a CL. For each Exposed heifer, an association index (with males) was determined thrice weekly, based on the distance to androgenized steers every 10 min for 4 h (until cyclic activity began). Data were analyzed according to heifer's initial body weight, which was categorized into three ranges (low, medium, and high, designated LW, MW, and HW, respectively). The cumulative proportion of cyclic heifers was greater for Exposed than Control heifers as of Day 21. By the end of the exposure period, more Exposed than Control heifers had attained puberty (16/66 vs. 2/65; P < 0.001). Within the HW classification, more Exposed than Control heifers became puberal (11/20 vs. 2/21; P = 0.002). However, there were no differences between MW and LW in the proportions of heifers that reached puberty. Association index in Exposed heifers was greater in HW than in MW and LW (0.10 +/- 0.09 vs. 0.06 +/- 0.03 and 0.06 +/- 0.04; P < 0.05), and in heifers that began cyclic activity compared to those that did not in the MW heifers (0.09 +/- 0.05 vs. 0.05 +/- 0.02; P = 0.01) and tended to be different in the HW treatment (0.12 +/- 0.10 vs. 0.06 +/- 0.02; P = 0.09). In conclusion, exposure of peri-puberal beef heifers to androgenized steers for 35 d advanced puberty in heavier heifers; an earlier response occurred in heifers with greater proximity to androgenized steers.